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2022 Vol.10, Issue 4 Preview Page

Research Article

Preparation and Characterization of Biosensor based on Zwitter Ion Polymer by Plasma Polymerization

플라즈마 중합에 의한 쯔비터 이온 고분자 기반의 바이오센서 제조 및 특성 평가

Jong-Sook Chon1, Eun-Ji Kim1, Chol Jung2, In-Kyoung Kim3, and Seong-Ho Choi4

천종숙1, 김은지1, 정철2, 김인경3, 최성호4

1Graduate Student, Department of Cosmetic Science, Hannam University
2Undergraduate Student, Department of Chemistry, Hannam University
3Professor, Department of Cosmetic Science Hannam University
4Professor, Department of Cosmetic Science and Chemistry Hannam University
1한남대학교 코스메틱사이언스학과 대학원생
2한남대학교 화학과 대학생
3한남대학교 코스메틱사이언스학과 교수
4한남대학교 코스메틱사이언스학과/화학과 교수
31 December 2022. pp. 45-54
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Abstract
본 연구에서는 비닐이미다졸(vinyl imidazole)과 1,3-프로페인(1.3-Propane), 설폰(sulfone)을 아세토니트릴 용액에 24시간 첨가반응시켜 쯔비터 이온 비닐단량체인 Sulfobetaine vinylimidazol(SBVI)을 합성하고, 쯔비터 이온 비닐단량의 성공적인 합성 여부는 1H-NMR, FT-IR과 질량분석을 통하여 확인할 수 있었다. 다음으로, 쯔비터 이온 비닐 단량체를 플라즈마 중합을 통해 ITO 전극 표면에 코팅 처리하여 쯔비터 이온 고분자 전극을 제조하였다. 쯔비터 이온 고분자 전극의 성공적인 합성 여부는 FT-IR, contact angle, SEM, AFM, Cyclic voltammetry를 통하여 확인할 수 있었다. 따라서, 쯔비터 이온 고분자 전극 표면에 GOx를 고정화시켜 글루코스 측정용 바이오센서를 제조하였다. 이 제조한 글루코스 측정용 바이오센서의 경우, 측정범위는 0.1mM~1.0mM이고, 센싱감도는 0.1mM, 안정성은 25회, L-cystein, ascobic acid, uric acid를 이용하여 방해효과를 측정한 결과 방해효과가 매우 낮은 글루코스 측정용 바이오센서가 제조됨을 확인할 수 있었다. 이러한 결과로서 쯔비터 이온 고분자의 경우, 효소기반 바이오센서를 제조하는 고정화 물질로 매우 유효함을 확인할 수 있었다.
In this study, we synthesized the vinyl monomer with zwitter ion named by sulfobetane vinyl imidazole, SBVI by addition reaction of vinyl imidazole and 1,3-propane, sulfone in acetonitrile for 24 hours. The successful synthesis of the SBVI was confirmed by analysis of 1H-NMR, FT-IR, GC-Mass, respectively. Next we prepared the zwitter ion polymeric electrode by plasma polymerization coating of the synthesized SBVI onto ITO glass, and then we also confirmed the successful preparation of zwitter ion polymeric electrode via surface analysis of FT-IR, contact angle, SEM, AFM and cyclic voltammetry analysis, respectively. Finally we fabricated the glucose bisosensor by immobilization of glucose oxidase, GOx, on the surface of zwitter ion polymeric electrode. The prepared glucose biosensor based on zwitter ion polymeric electrode was to have sensing range of 0.1 mM~1.0 mM, limiting sensing of 0.1 mM, stability with 25 cycles the low interference effects for L-cystein, ascobic acid, and uric acid. As a result, the zwitter ion polymeric electrode is confirmed one of the useful materials for fabrication of biosensor.
Keywords
Plasma polymerization
Zwitter ion polymer
Biosensor for detection of glucose
Interference effects
Sulfobetaine vinylimidazole
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Information
  • Publisher :The Society of Convergence Knowledge
  • Publisher(Ko) :융복합지식학회
  • Journal Title :The Society of Convergence Knowledge Transactions
  • Journal Title(Ko) :융복합지식학회논문지
  • Volume : 10
  • No :4
  • Pages :45-54
  • DOI :https://doi.org/10.22716/sckt.2022.10.4.035
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